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ea0032p991 | Thyroid (non-cancer) | ECE2013

Quantification of tissue T3 and T4 in rat and human heart by a novel HPLC-MS/MS method

Saba Alessandro , Colligiani Daria , Nannipieri Monica , Kusmic Claudia , Dos Remedios Cristobal , Simonides Warner , Iervasi Giorgio , Zucchi Riccardo

Thyroid hormone (T3) is mostly produced in peripheral tissues by thyroxine (T4) deiodination, and its functional effects are directly related to tissue concentration, which is quite difficult to assay by conventional immunological methods. We report a novel technique, based on tandem mass spectrometry (MS/MS) coupled to HPLC, which was used in left ventricular myocardium obtained from rat and human hearts.Samples were homogenized in...
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Published by BioScientifica

Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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